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. 2026 Jan 23;16:1724684. doi: 10.3389/fimmu.2025.1724684

Figure 8.

Three panels on the left depict areas with dotted outlines labeled “n.i.,” “WT,” and “ΔdotA,” showing varying surface distributions. The right panel is a bar graph comparing the resorbed surface percentage across “n.i.,” “WT,” and “ΔdotA” groups, with ΔdotA showing the highest resorption. Significant differences are marked with p-values: <0.0001 and 0.0113.

C. burnetii infection of osteoclasts inhibits their bone resorption activity in a DotA dependent manner. The degradation activity of osteoclasts was measured with cells seeded on a collagen embedded plate using a differentiation and infection protocol similar to Figure 1A. Infection was realized on day 2 of differentiation. Degradation activity was analyzed on day 7 (5 dpi). Plates were imaged by light microscopy. Area of collagen degradation (resorption pit) was quantified with ImageJ (3 mice for each condition; 5 wells per mice and condition). Scale bar represents 100 μm. Data are shown as mean ± SD. Circle represent individual microscopical fields of view.