TABLE 3.

Measured kinetic parameters of Ema1 to Ema16^a

CYP	Km (μM)	Vmax (μmol min−1 mg−1)	kcat (s−1)	kcat/Km (M−1 s−1)
Ema1	4.3 ± 2.3	0.060 ± 0.009	0.048	11.1 × 103
Ema2/14	3.7 ± 3.1	0.014 ± 0.003	0.011	3.1 × 103
Ema3	6.9 ± 8.4	0.013 ± 0.005	0.011	1.6 × 103
Ema4/10	4.4 ± 2.6	0.043 ± 0.007	0.034	7.8 × 103
Ema5	2.2 ± 4.1	0.023 ± 0.008	0.018	8.2 × 103
Ema6	1.1 ± 0.7	0.014 ± 0.002	0.011	10.3 × 103
Ema7	2.3 ± 2.8	0.005 ± 0.001	0.004	1.7 × 103
Ema8	2.6 ± 1.4	0.007 ± 0.001	0.006	2.4 × 103
Ema9	4.3 ± 2.3	0.021 ± 0.003	0.017	3.9 × 103
Ema11		0.001 ± 0.001	0.001
Ema12	2.7 ± 2.8	0.012 ± 0.003	0.010	3.5 × 103
Ema13	6.5 ± 3.3	0.053 ± 0.006	0.042	6.6 × 103
Ema15	2.6 ± 1.7	0.034 ± 0.005	0.027	10.4 × 103
Ema16	3.8 ± 3.6	0.008 ± 0.002	0.006	1.7 × 103

^aThe values were determined as described in Materials and Methods. Ema2/Ema14 and Ema4/Ema10 are identical by their respective amino acid sequences.