FIG. 2.

Agar-based biocatalytic conversion assays with S. lividans ZX7 strains. Avermectin to 4"-oxo-avermectin end-point bioconversion assays were performed in triplicate with strains growing on avermectin-containing agar plates as described in Materials and Methods. S. lividans ZX7 carried different expression vector-ema gene-fd (ferredoxin) gene combinations as shown below the bars. Gene versions for ema: 1, ema1; 1A, ema1A; 1CTG, ema1CTG; 2, ema2; 2CTG, ema2CTG. Fd-encoding genes: 232, fd232; 233, fd233; none, no FD-encoding gene was included in the construct.