TABLE 2.
S-protein gene-specific oligonucleotides used to investigate the prevalence of different genes among L. gallinarum isolates
| Gene | Primer | Sequence (5′ to 3′) | Ta (°C) used for screening PCR | Size of amplified fragment (kb) |
|---|---|---|---|---|
| lgsA | Fsl-1 | CATCAAACAATACTGTTACAAAC | 51 | 0.34a |
| Rsl-1 | CAAACCATTAGCATCAATATC | |||
| lgsB | Fsl-2 | GATAACGGATCACACTTTGG | 53 | 0.33a |
| Rsl-2 | AGGTTGTTAATGTTAGCTGTAG | |||
| lgsC | Fsl-3 | CAAAGGCTGAAATGACTACTAC | 57 | 0.6a |
| Rsl-3 | CTTAGCATTGTCAACATGTACG | |||
| lgsE | Fsl-5 | CAACTTGACAAGAATGGTACTG | 57 | 0.26 |
| Rsl-5 | GTACTTGATGTCACTTGATTCC | |||
| lgsF | D109E-2 | CATTAACTTGGGTGGTTCAGACG | 63 | 0.22a |
| D109E-3 | TACCGCCTTGTACAGCAGTAACC | |||
| lgsG | Fsl-6 | GTTACTATCACTGTTCCAGCAGG | 63 | 0.30 |
| Rsl-6 | CTACGTAGTTCTTATCAAATTCAG | |||
| lgsH | Fsl-7 | GGGCTGCACATAATGCGACTGA | 63 | 0.28 |
| Rsl-7 | GAAATAGCACTTGCATCTGAGCC | |||
| D42C-3 | ATACTAAGGCTGACTCAGTAG | 64 | 0.58 | |
| Rsl-7 | GAAATAGCACTTGCATCTGAGCC | |||
| lgsI | P5E-1 | TAGCTGATGCTGCACAAGACG | 59 | 0.52 |
| P5E-3 | ACTAGCATCAACATCAACACCG |
a
The expected sizes of the amplified products for the primers used in the clean-room experiments are indicated.