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Multistep oxygenation of m-nitrotoluene by E. coli JM101(pSPZ3). The assay was performed with resting cells (1.7 g CDW/liter) as described in Materials and Methods. m-Nitrotoluene at 1.0 mM (⧫) served as a substrate. m-Nitrobenzyl alcohol (▪), m-nitrobenzaldehyde (▴), and m-nitrobenzoic acid (•) were formed as consecutive products. ×, sum of nitroaromatic reactant concentrations.
