TABLE 3.
Formation of aromatic amines from corresponding nitroaromatics
| Strain | Aromatic amine formation rate (U/g CDW) ona:
|
|||
|---|---|---|---|---|
| m-Nitrotoluene | m-Nitrobenzyl alcohol | m-Nitrobenzaldehyde | m-Nitrobenzoic acid | |
| E. coli JM101 | 1.18 ± 0.04 | 0.70 ± 0.11 | 2.63 ± 0.24 | 3.39 ± 0.32 |
| P. putida DOT-T1E | 0.14 ± 0.03 | 0.10 ± 0.03 | 0.55 ± 0.06 | 0.55 ± 0.07 |
| P. putida PpS81 | 0.09 ± 0.07 | 0.02 ± 0.02 | 0.15 ± 0.06 | 0.09 ± 0.05 |
a
Formation rates (means ± standard deviations) are based on aromatic amine concentrations detected by Ehrlich's reagent in whole-cell activity assays using resting cells (≈2 g CDW/liter). See Materials and Methods for details. One unit is the enzyme activity that forms 1 μmol of product per min.