Agarose gel electrophoresis. (A) PFGE of replicon DNA. Lane 1, the three replicons of strain Rm1021; lane 2, the three replicons of strain ATCC 9930. (B) Gel electrophoresis of RDA subtractive products. Lane M, 1-kb size standard; lane 1, subtractive products from the first round of RDA; lane 2, subtractive products from the second round of RDA.