FIG. 6.

PCR products from genomic DNA samples amplified using the primer combination 27F and alpha303R (A) or 27F and ins63bpR (B) on 2% agarose gels. Lane 1 contains a 100-bp DNA ladder (New England Biologicals), while the remaining lanes contain the PCR products from the following sponges: lanes 2 and 3, Lendenfeldia chondrodes; lane 4, P. papyracea; lane 5, Lamellodysidea herbacea; lane 6, Lamellodysidea chlorea; lane 7, no-DNA negative controls. All PCR products shown were amplified from samples collected at site 1 except for Lamellodysidea chlorea, which was from site 4. All samples collected in this study were surveyed, and the patterns of Lamellodysidea herbacea and P. papyracea from the other sites were identical to the representative sample for the species (not shown).