TABLE 1.
PCR results for San Diego samples and controls
| Identifiera | Speciesb (no. of ticks) | PCR assay resultse
|
||
|---|---|---|---|---|
| San Diego 16S rRNA gene standard PCR | F11-F5 16S rRNA gene standard PCR | Multitarget TaqMan assay (ISFtu2, tul4, and iglC) | ||
| 2022859c | Sylvilagus audoboni | + | ND | + |
| 2032026c | Sylvilagus audoboni | + | ND | + |
| 2030223 | D. variabilis (1) | + | ND | − |
| 2030224 | D. variabilis (1) | + | + | − |
| 2030362 | D. variabilis (7) | + | ND | − |
| 2030363 | D. variabilis (1) | + | ND | − |
| 2030997 | D. variabilis (1) | + | + | − |
| 2040278 | D. variabilis (2) | + | ND | − |
| 2040369 | D. variabilis (11) | + | + | − |
| 2040460d | D. variabilis (1) | + | + | − |
| 2040445 | D. variabilis (4) | + | + | − |
| 2040409 | D. variabilis (1) | + | + | − |
| 2040384d | D. variabilis (1) | + | + | − |
| 2040372 | D. variabilis (12) | + | + | − |
| 2040370 | D. variabilis (3) | + | + | − |
| 2040371 | D. variabilis (10) | + | + | − |
| 2032437 | D. occidentalis (9) | + | + | − |
| 2031093d | D. occidentalis (1) | + | ND | − |
| 2032666 | Canine (1) | + | + | − |
| 2040006 | Canine (1) | + | + | − |
| 2040431 | Cat (1) | + | + | − |
| 2040386 | Mountain lion (1) | + | + | − |
| 2032922 | Coyote (1) | + | + | − |
| MV1d | D. variabilis (1) | ND | + | − |
| MV2 | D. variabilis (1) | ND | ND | − |
| MV3 | D. variabilis (1) | ND | ND | − |
| MV1 (spiked) | ND | ND | + | |
a
The identifier for the first 23 samples corresponds to the accession number assigned at the San Diego Animal Disease Diagnostic Laboratory.
b
Ticks whose species was not identified are referred to by source.
c
F. tularensis-positive rabbits.
d
Samples subjected to 16S rRNA gene sequencing.
e
ND, not determined.