TABLE 3.
Taxonomic affiliations of representative clones from the RFLP groups for the clone libraries from BG, determined by phylogenetic analysis of 16S rRNA gene sequences
| Representative clone(s)a | No. of clones | Phylogenetic clusterb | Nearest neighborc (accession no.) | % Similarityd | Comment |
|---|---|---|---|---|---|
| BG.A3, BG.F8 | 4 | Alphaproteobacteria | Rhizobium sp. strain RM1-2001 (AF331662) | 97 | Isolated from arsenic-contaminated mine tailings |
| BG.B9, BG.H6 | 23 | Betaproteobacteria (“Gall217”) | JG36-GS-54 (AJ582038) | 95 | Heavy-metal-contaminated environment clone |
| BG.G12, BG.F2 | 3 | Betaproteobacteria (“Gall217”) | GOUTA12 (AY050584) | 95 | Monochlorobenzene-contaminated groundwater clone |
| BG.A7, BG.A8, BG.E7, BG.F11 | 19 | Betaproteobacteria (“Com1212”) | Delftia acidovorans (AF181575) | 99 | Related to S0-oxidizing isolates |
| BG.D1 | 3 | Betaproteobacteria (“Com1212”) | PRD01b008B (AF289168) | 97 | Freshwater bacterial clone |
| BG.D11 | 1 | Betaproteobacteria (“Com1212”) | Rhodoferax ferrireducens (AF435948) | 98 | Psychrotolerant, facultatively acetate-oxidizing, Fe(III)-reducing isolate |
| BG.B2 | 3 | Betaproteobacteria (“Com1212”) | Rhodoferax ferrireducens (AF435948) | 96 | Psychrotolerant, facultatively acetate-oxidizing, Fe(III)-reducing isolate |
| BG.C1 | 7 | Betaproteobacteria | P3OB-54 (AF414582) | 98 | U(VI)-reducing community clone |
| BG.H11, BG.B1 | 5 | Betaproteobacteria | Burkholderia cepacia LMG12614 (AF311970) | 99 | Isolate from cystic fibrosis sputum |
| BG.G3 | 4 | Gammaproteobacteria | Stenotrophomonas maltophilia strain e-a21 (AJ293470) | 98 | Isolate from sewage treatment plant |
| BG.D4 | 4 | Gammaproteobacteria | Pseudomonas synxantha (AF267911) | 99 | Isolated from pine tree buds |
| BG.A10 | 2 | Gammaproteobacteria | HTB10 (AF418945) | 94 | Freshwater aquifer metal-rich particle clone |
| BG.C4 | 3 | Deltaproteobacteria | Hyd24-27 (AJ535224) | 90 | Marine sediment clone |
| BG.B12, BG.B6 | 10 | Epsilonproteobacteria | RA9C8 (AF407391) | 99 | Monochlorobenzene-contaminated groundwater clone |
| BG.C5 | 1 | CFB | GKS2-106 (AJ290025) | 98 | Lake Gossenkoellesee clone |
| BG.F6 | 1 | Holophaga/Acidobacteria | DS-18 (AY289393) | 91 | Soil clone |
| BG.H3 | 1 | Spirochaeta | Leptospira fainei (Y19243) | 91 | Isolated from human patient with Weil's disease |
a
Fully, bidirectionally sequenced clones representing HhaI RFLP groupings.
b
Phylum-level grouping. Names in parentheses are groupings shown in Fig. 2.
c
Most closely related sequence in the GenBank public database, based upon BLASTN search.
d
Percent similarity to nearest neighbor, based upon BLASTN search.