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Zymogram of soluble acceptor:MV hydrogenases among the deep-sea Epsilonproteobacteria. Each cell extract (100 μg protein for lanes 1 to 7) was run in a 4 to 20% (wt/vol) gradient native polyacrylamide gel. Lanes: 1, H. thermophila; 2, “S. paralvinella”; 3, S. autotrophica; 4, L. acidiphila; 5, N. salsuginis; 6, S. lithotrophicum; 7, T. micantisoli; 5′, N. salsuginis (500 μg protein); M, marker (Kaleidoscope prestained standards). Arrowheads indicate the visualized hydrogenase bands.
