FIG. 4.

Transcriptional activity from pltB::inaZ in the wild-type Pf-5 background (a), pltJ::inaZ in the wild-type Pf-5 background (b), pltJ::inaZ in a pltF background (c), and pltJ::inaZ in a pltR background (d). The parental strain Pf-5 (Ina⁻; triangles) was included as a negative control (a). Cultures were grown in NBGly with (open symbols) or without (filled symbols) an amendment of 4 μg/ml pyoluteorin added at the time of inoculation and were harvested at 0, 2, 12, 24, and 48 h for assessments of INA (a to d) and population densities (e to h). Error bars denote 1 standard error.