TABLE 1.
Bacterial strains and plasmids used for this study
| Strain genotype or plasmid | Strain no. | Descriptiona | Reference |
|---|---|---|---|
| Strains | |||
| P. fluorescens | |||
| Pf-5 | JL4474 | Rhizosphere isolate | 18 |
| Pf-5 pltB::inaZ | JL4389 | pltB::Tn3-nice derivative of Pf-5; Plt− Ina+ Kmr | 23 |
| Pf-5 pltR::aacC1 | JL4563 | pltR::aacC1 derivative of Pf-5; Plt− Gmr | 32 |
| Pf-5 pltF::aacC1 | JL4629 | pltF::aacC1 derivative of Pf-5; Plt− Gmr | This study |
| Pf-5 pltJ::inaZ | JL4671 | pltJ::inaZ derivative of Pf-5; Ina+ Kmr | This study |
| Pf-5 pltI::inaZ | JL4673 | pltI::inaZ derivative of Pf-5; Ina+ Kmr | This study |
| Pf-5 pltR::aacC1 pltJ::inaZ | JL4677 | pltR::aacC1 pltJ::inaZ derivative of Pf-5; Ina+ Gmr Kmr | This study |
| Pf-5 pltR::aacC1 pltI::inaZ | JL4679 | pltR::aacC1 pltI::inaZ derivative of Pf-5; Ina+ Gmr Kmr | This study |
| Pf-5 pltF::aacC1 pltJ::inaZ | JL4683 | pltF::aacC1 pltJ::inaZ derivative of Pf-5; Ina+ Gmr Kmr | This study |
| Pf-5 pltF::aacC1 pltI::inaZ | JL4685 | pltF::aacC1 pltI::inaZ derivative of Pf-5; Ina+ Gmr Kmr | This study |
| E. coli | |||
| S17-1 | Res− Mod+recA Tra+ | 40 | |
| DH5α | F−endA1 hsdR17 (rK− mK+) supE44 thi-1 recA1 gyrA96 relA1 φ80dlacZΔM15 λ− | 36 | |
| Plasmids | |||
| pRK415 | IncP1 replicon, polylinker of pUC19; Mob+ Tcr | 21 | |
| pRK2013 | Mobilizing plasmid; Tra+ Kmr | 11 | |
| pUC19 | ColE1 replicon; Apr | 36 | |
| pTn3-nice | pMB8 replicon, contains Tn3-nice; Apr Kmr | 23 | |
| pJEL1938 | 29 kb of the pyoluteorin biosynthetic gene cluster from Pf-5 cloned into pLAFR3; Tcr | 23 | |
| pJEL6198 | 1.7-kb PstI fragment containing pltF cloned from pJEL1938 into pUC19; Apr | This study | |
| pJEL6199 | pltF::aacC1 in pUC19; Apr Gmr | This study | |
| pJEL6200 | pltF::aacC1 in pRK415; Tcr Gmr Mob+ | This study | |
| pJEL6274 | 3.4-kb SmaI-HindIII fragment from pJEL1938 encompassing pltI and pltJ, cloned into pUC19; Apr | This study | |
| pJEL6279 | 6.2-kb fragment containing the inaZ reporter gene and nptI from pTn3-nice, cloned into pUC19; Apr Kmr Ina+ | 6 | |
| pJEL6285 | pltJ::inaZ fusion (at SphI site of pltJ) in pUC19; Kmr Apr | This study | |
| pJEL6287 | pltI::inaZ fusion in pUC19; Kmr Apr | This study | |
| pJEL6299 | pltJ::inaZ fusion in pRK415; Kmr Tcr Mob+ | This study | |
| pJEL6300 | pltI::inaZ fusion in pRK415; Kmr Tcr Mob+ | This study |
a
Apr, Gmr, Kmr, and Tcr indicate resistance to ampicillin, gentamicin, kanamycin, and tetracycline, respectively. Plt− indicates a lack of pyoluteorin production. Ina+ indicates ice nucleation activity at −5°C.