TABLE 1.
Strains, plasmids, and PCR primers used in this study
| Strain, plasmid, or primer | Description and/or relevant genotypea | Reference or source |
|---|---|---|
| R. solanacearum strains | ||
| GMI1000 | Wild-type strain | 29 |
| R3 | GMI1000, spontaneously Rifr | This study |
| R3-1501− | R3::pCN15, Kmr | This study |
| R3-337− | R3::pCN337, Kmr | This study |
| R3-1530− | R3::pCN1530, Kmr | This study |
| E. coli strains | ||
| DH5α | Invitrogen | |
| S17-1 (λpir) | Tpr Smr, recA thi hsdRM+, λpir phage lysogen RP4::Mu::Km Tn7 | 9 |
| Plasmids | ||
| pFSV | Cmr Tcrori R6K mob RP4 | 4 |
| pFSVIN3 | Cmrori R6K mob RP4 ppoA::mini-Tn10 Gmr | 21 |
| pFSVCN | ori R6K mob RP4 ′ppoA′ Gmr | 21 |
| pFSVK | ori R6K mob RP4 ′ppoA′ Kmr | 21 |
| pBRI15 | pBKSII + 180-bp BamHI internal fragment from RSc1501 gene | This study |
| pCN1501 | pFSVK + 235-bp EcoRI-SacI fragment from pBRI15 | This study |
| pCN337 | pFSVK + 300-bp NcoI-SacI fragment from RSc0337 amplified by PCR | This study |
| pCN1530 | pFSVK + 800-bp SacI-EcoRI fragment from RSp1530 amplified by PCR | This study |
| Primers | ||
| F1501 | GTTTTCGAATTCTGCCTGACG (EcoRI) | |
| FI337 | TCGCCATGCCGTACTGGAAC (NcoI) | |
| FI1530 | ACGGCGAGCTCGACACCCTG (SacI) | |
| R1501 | GGTGGTGAGCTCCCAATGAAG (SacI) | |
| RI337 | GCGTGAGCTCCAGGATGC (SacI) | |
| RI1530 | ACGGTGAATTCGAACGCGTCG (EcoRI) |
a
Engineered restriction sites are underlined.