TABLE 2.
Plasmid used to transform JM109 cells | Chloramphenicol MIC (mg/liter) | CAT level (pg/μg)a |
---|---|---|
None | 8 | 4 ± 0.5 |
pKK232-8b | 8 | 4 ± 0.5 |
pKK232-8 (ATCC 25922)c | 32 | 120 ± 32 |
pKK232-8 (99004202)d | 512 | 761 ± 41 |
pKK232-8 (96010266M)e | 32 | 95 ± 8 |
Mean ± standard deviation of three determinations of CAT concentration.
pKK232-8 without any insert.
pKK232-8 containing the ampC promoter from strain ATCC 25922 (low-level AmpC producer).
pKK232-8 containing the ampC promoter from strain 99004202 (moderate-level AmpC producer).
pKK232-8 containing the ampC promoter from the mutant 96010266M (3).