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Validation curves plotting actual tet gene copies versus quantified tet gene copies by real-time PCR assays. (A) tet(A); (B) tet(G); (C) RPP tet genes. The actual numbers of tet gene copies (x axis) were plotted against the quantification values (y axis) for the tet genes (solid lines). Theoretical plots assume 100% accuracy (dashed lines). Error bars (both x and y) indicate standard deviations (n = 3).
