TABLE 1.

PCR primer sequences, targets, annealing temperatures, and amplicon lengths

Primer	Class targeted	Primer sequence (5′→3′)	Primer annealing temp (°C)c	Amplicon size (bp)	Reference
tetAC-150fa	Tet A, C	GCT RTA TGC GTT GRT GCA AT	58	567	This study
tetAC-716ra	Tet A, C	TCC TCG CCG AAA ATG ACC
tetG-247fb	Tet G	GTC GAT TAC ACG ATT ATG GC	57	432	This study
tetG-678rb	Tet G	CAC TTG GCC GAT CAG TTG A
Ribo2-FW	Tet M, O, P, Q, S, T, W	GGM CAY RTG GAT TTY WTI GC	52	1,315	5
Ribo2-RV	Tet M, O, P, Q, S, T, W	TCI GMI GGI GTR CTI RCI GGR C

^aNumbered according to the tet(A) gene in plasmid pRP1 in E. coli (GenBank accession no. X00006).

^bNumbered based on the tet(G) gene in plasmid pSTG1 in a Pseudomonas sp. (GenBank accession no. AF133139).

^cFor touchdown PCR. See the text for details.