FIG. 2.

Time dependence of l-Leu-pNA hydrolysis (A), l-Leu-NH₂ hydrolysis (B), and l-Leu-O-Me hydrolysis (C) by wild-type SSAP. (A) Continuous spectrophotometric assay for l-Leu-pNA hydrolysis. The y axis shows the amount of released p-nitroaniline determined from the increase in absorbance at 405 nm. (B) Time dependence of l-Leu-NH₂ hydrolysis. (C) Continuous spectrophotometric assay of l-Leu-O-Me hydrolysis. The y axis shows the absorbance at 230 nm. The values are averages of three independent experiments. In all cases, the standard deviation was less than 5% of the mean.