TABLE 2.
l-Serine accumulation of various C. glutamicum strains
| Strain |
l-Serine concn (mM)a at:
|
|
|---|---|---|
| 28 h | 54 h | |
| WT | <0.01 | <0.01 |
| WT(pZ1)(pEC-T18mob2) | <0.01 | <0.01 |
| WT(pZ1serA)(pEC-T18mob2) | <0.01 | 0.12 ± 0.10 |
| WT(pZ1serAΔ197)(pEC-T18mob2) | <0.01 | 0.08 ± 0.05 |
| WT(pZ1)(pEC-T18mob2serCB) | <0.01 | 0.04 ± 0.03 |
| WT(pZ1serA)(pEC-T18mob2serCB) | <0.01 | 0.11 ± 0.03 |
| WT(pZ1serAΔ197)(pEC-T18mob2serCB) | <0.01 | 0.05 ± 0.02 |
| WT ΔsdaA(pZ1)(pEC-T18mob2) | 0.08 ± 0.03 | 0.05 ± 0.01 |
| WT ΔsdaA(pZ1serA) (pEC-T18mob2serCB) | 0.09 ± 0.01 | 0.15 ± 0.01 |
| WT ΔsdaA(pZ1serAΔ197) (pEC-T18mob2serCB) | 0.44 ± 0.28 | 0.14 ± 0.01 |
a
l-Serine accumulation was determined on minimal medium CGXII with 220mM glucose in two or three independent experiments.