TABLE 1.
Hydrolyzing activities of Chi1 and mutants of this enzyme
| Enzyme | Specific hydrolyzing activity (U/μmol protein)
|
|||
|---|---|---|---|---|
| Crystalline chitin | Colloidal chitin | Soluble chitin | pNP-chitobiose | |
| Chi1 | 5.24 ± 0.22 (100)a | 68.55 ± 2.83 (100) | 1,315.0 ± 54.2 (100) | 48.66 ± 5.91 (100) |
| W70A | 1.91 ± 0.13 (37) | 42.84 ± 2.72 (62) | 1,328.2 ± 50.1 (101) | 53.52 ± 6.82 (110) |
| S33A | 1.92 ± 0.11 (37) | 40.44 ± 2.06 (59) | 1,354.5 ± 57.8 (103) | 49.15 ± 5.73 (101) |
| W245A | 1.93 ± 0.15 (37) | 30.02 ± 2.31 (44) | 1,275.6 ± 55.7 (97) | 45.74 ± 5.88 (94) |
| W232A | 3.02 ± 0.18 (58) | 40.78 ± 1.78 (60) | 1,341.3 ± 60.5 (102) | 58.39 ± 4.91 (120) |
| S33W | 2.86 ± 0.19 (55) | 40.44 ± 2.51 (59) | 1,354.5 ± 50.4 (103) | 62.28 ± 7.12 (128) |
| Chi1ΔChBD | 1.73 ± 0.09 (33) | 51.41 ± 2.56 (75) | 1,301.9 ± 48.1 (99) | 48.64 ± 5.56 (100) |
a
The values are means ± standard deviations for three independent experiments. The values in parentheses are relative specific activities (expressed as percentages).