FIG. 7.

HPLC chromatogram for broth samples. The solid line represents the standard solutions used: glucosamine at 15 mM and glucose at 13.5 mM. The short-dashed line represents a broth sample (1:10 dilution) taken at 24 h of fermentation (50 g/liter initial glucose concentration). The long-dashed line represents a broth sample (1:100 dilution) taken at 48 h of fermentation (150 g/liter initial glucose concentration). The peaks obtained at about 8 min retention time correspond to glucosamine, while those at 12.5 min correspond to glucose.