TABLE 2.
Total numbers of Cryptosporidium oocysts in water samples from the Rhine River and the Meuse River as determined by immunofluorescence microscopya
| Sampling date (day/mo/yr) | River | Total no. of oocysts | No. of potentially infectious oocysts
|
Cell line | Cell culture infectivity | |
|---|---|---|---|---|---|---|
| DAPI+ PI− | DAPI− PI− | |||||
| 17/01/00 | Meuse | 4 | 0 | 4 | Caco-2 | + |
| 24/01/00 | Meuse | 6 | 6 | 0 | Caco-2 | + |
| 07/02/00 | Meuse | 2 | 2 | 0 | Caco-2 | − |
| 21/02/00 | Meuse | 10 | 0 | 5 | HCT-8 | − |
| 28/02/00 | Meuse | 16 | 1 | 6 | HCT-8 | − |
| 13/03/00 | Meuse | 25 | 2 | 12 | Caco-2 | − |
| 08/05/00 | Meuse | 6 | 0 | 2 | HCT-8 | + |
| 15/05/00 | Meuse | 0 | 0 | 0 | HCT-8 | − |
| 28/02/00 | Rhine | 13 | 1 | 7 | HCT-8 | − |
| 13/03/00 | Rhine | 9 | 1 | 2 | Caco-2 | − |
| 27/03/00 | Rhine | 2 | 0 | 2 | HCT-8 | − |
| 03/04/00 | Rhine | 4 | 0 | 2 | HCT-8 | − |
| 10/04/00 | Rhine | 5 | 1 | 3 | HCT-8 | − |
| 08/05/00 | Rhine | 18 | 0 | 6 | HCT-8 | − |
| 05/06/00 | Rhine | 7 | 0 | 3 | HCT-8 | − |
| 13/03/00 | Meuse (spiked) | 19 | 2 | 13 | Caco-2 | − |
| 15/05/00 | Meuse (spiked) | 245 | 0 | 240 | HCT-8 | + |
| 27/03/00 | Rhine (spiked) | 9 | 0 | 7 | HCT-8 | − |
| 08/05/00 | Rhine (spiked) | 159 | 9 | 126 | HCT-8 | + |
a
Potentially infectious oocysts excluded propidium iodide and contained sporozoites. The samples produced infection (+) or did not produce infection (−) in Caco-2 or HCT-8 cells in cell culture infectivity assays.