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Determination of LC50s. Representative results obtained for the reference strain, ATCC 9545, and strains 03-189 and 01-440 are shown. Mortality due to AFB was determined as described in the text, and the percentage of AFB-dead larvae was calculated for each concentration (100% = 30 larvae). Experiments were performed three times. The mean values ± standard deviations of mortality are plotted against the spore concentrations to determine the strain-specific LC50s.
