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The effect of hydrogen concentration on the quantity of tceA genes (dashed lines, left y axis) and tceA transcripts per tceA gene (solid lines, right y axis). Parallel ANAS subcultures were amended with TCE and differing masses of hydrogen to obtain aqueous hydrogen concentrations of 12 nM (▴), 220 nM (□), or 17 μM (▪). All tceA measurements are averages of triplicate qPCRs or triplicate RT-qPCRs, and error bars represent 1 standard deviation.
