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Effect of pH on activity of M. bovis haloalkane dehalogenases DmbA (top panel) and DmbB (bottom panel). The data for DmbA are expressed as relative activities, and error bars represent the standard errors. Experiments with DmbB were not replicated due to insufficient protein material. Measurements were made with the substrate 1,2-dibromoethane at 37°C in 100 mM buffers: potassium acetate (•), potassium phosphate (▪), or glycine buffer (▴).
