FIG. 5.

Effect of temperature on activity of haloalkane dehalogenases DmbA (top panel) and DmbB (bottom panel). The activities were determined with the substrate 1,2-dibromoethane. 2-Bromoethanol was identified as a reaction product by using gas chromatography. The data for DmbA are expressed as relative activities, and error bars represent the standard errors. Experiments with DmbB were not replicated due to insufficient protein material. The specific activity of DmbA under optimal conditions is 0.2125 μmol s⁻¹ mg of protein⁻¹; the specific activity of DmbB is 0.0044 μmol s⁻¹ mg of protein⁻¹.