TABLE 2.
Comparison of kinetic constants determined for wild-type LinB, sixfold LinB mutant, and wild-type DmbA
| Substrate | Mean ± SE
|
kcat/Km (mM−1 s−1) | ||
|---|---|---|---|---|
| Km (mM) | Ksi (mM) | kcat (s−1) | ||
| LinB | ||||
| 1-Chlorobutane | 0.23 ± 0.02 | NAa | 1.11 ± 0.03 | 4.83 |
| 1,3-Dibromopropane | 24.1 ± 3.23 | 0.49 ± 0.06 | 40.9 ± 5.2 | 1.70 |
| Sixfold mutant LinB | ||||
| 1-Chlorobutane | 0.14 ± 0.02 | NA | 0.58 ± 0.003 | 4.14 |
| 1,3-Dibromopropane | 10.07 ± 2.44 | 1.63 ± 0.34 | 21.50 ± 2.8 | 2.13 |
| DmbA | ||||
| 1-Chlorobutane | 0.16 ± 0.04 | NA | 0.08 ± 0.004 | 0.50 |
| 1,3-Dibromopropane | 4.52 ± 0.71 | 2.65 ± 0.49 | 9.20 ± 1.17 | 2.03 |
a
NA, not applicable.