TABLE 3.
Nitrogen content and dry weight of Vigna unguiculata plants inoculated with the Bradyrhizobium sp. (Vigna) wild-type M5 and hupSL mutant M5.1 strains in three independent experimentsa
| Experiment | Strain | 30 days
|
40 days
|
||
|---|---|---|---|---|---|
| N content | Dry weight | N content | Dry weight | ||
| I | M5 | 58.4a | 1.31a | 81.4a | 2.48a |
| M5.1 | 40.7b | 1.24a | 74.3b | 2.46a | |
| Uninoculated | 6.11c | 0.48b | 2.91c | 0.56b | |
| II | M5 | 46.7a | 1.25a | 113.1a | 3.64a |
| M5.1 | 30.2b | 0.94b | 102.4b | 4.71a | |
| Uninoculated | 6.05c | 0.43c | 2.53c | 0.61b | |
| III | M5 | ND | ND | 81.1a | 3.31a |
| M5.1 | ND | ND | 70.2b | 3.02a | |
| Uninoculated | ND | ND | 2.35c | 0.49b | |
Determinations were carried out after 30 or 40 days of cultivation. Nitrogen content (determined with a Kjeldhal apparatus) is expressed as mg plant−1 and dry weight (measured after drying for 48 h at 80°C) in g plant−1. Values are averages of eight determinations. Different letters within the same column and experiment indicate significant differences at 1% based on Duncan's test. ND, not determined.