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. 2005 Nov;71(11):7196–7202. doi: 10.1128/AEM.71.11.7196-7202.2005

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FIG. 4. — A. Agarose gel of PCR samples. Lane 1, wt M. hemolytica; lane 2, M. hemolytica aroA mutant (templates were amplified using the MharoA primer pair). Lane 3, M. hemolytica transformed with replacement plasmid; lane 4, M. hemolytica aroA mutant (templates were amplified with the Kan^r primer set). Lane 5, M. hemolytica transformed with replacement plasmid; lane 6, M. hemolytica aroA mutant (templates were amplified with the pD70ori primer set). B. Lane 1, wt P. multocida; lane 2, P. multocida aroA mutant (templates were amplified with the PmaroA primer set). Lane 3, P. multocida transformed with replacement plasmid; lane 4, P. multocida aroA mutant (templates were amplified with the Kan^r primer set). Lane 5, P. multocida transformed with replacement plasmid; lane 6, P. multocida aroA mutant (templates were amplified with the pD70ori primer set). C. Lane 1, wt H. somnus; lane 2, H. somnus aroA mutant (templates were amplified with the HsaroA primer set). Lane 3, H. somnus transformed with replacement plasmid; lane 4, H. somnus aroA mutant (templates were amplified with the Kan^r primer set). Lane 5, H. somnus transformed with replacement plasmid; lane 6, H. somnus aroA mutant (templates were amplified with the pD70ori primer set).