TABLE 1.

Recovery of the radiolabeled PIs following incubation in U937 cells^a

Treatment stepb	SQV	RTV	IDV
RPMI medium	86.5 ± 6.51	77.5 ± 0.84	81.3 ± 5.42
Wash 1	4.45 ± 1.92	8.04 ± 0.85	4.63 ± 2.10
Wash 2	1.90 ± 0.56	3.73 ± 0.57	2.77 ± 1.46
Wash 3	0.82 ± 0.17	2.02 ± 1.30	1.83 ± 0.54
60% Methanol	5.71 ± 0.64	8.37 ± 0.43	9.34 ± 2.49
Cell pelleting	0.57 ± 0.34	0.32 ± 0.09	0.18 ± 0.14

^aRadiolabeled PIs were administered in 1 μM concentrations following an 18-h incubation in 10 ml of 10⁶ U937 cells/ml. Data are expressed in percentages as means ± SD (n ≥ 4).

^bRecovery was from the supernatants of RPMI and washes in PBS.