TABLE 2.

Reproducibility and number of types obtained by using various DNA typing methods for differentiation of 90 M. tuberculosis complex strains and 10 non-M. tuberculosis complex mycobacterial strains

DNA target	Method used (ref.)	% Reproducibilitya (95% CI)b	No. of types obtained	No. of nontypeable TB complex strains	No. of non-M. tuberculosis complex strains positive
12 MIRUs	VNTR typing (65)	100 (89-100)	78	0	0
IS6110	FLiP	97 (83-100)	81	2	0
DR locus	Second-generation spoligotyping (71)	90 (74-98)	61	0	0
Five QUBsc	VNTR typing	87 (70-96)	82	0	0
IS6110	LM-PCR	81 (63-93)	73	2	0
Four conserved loci	Amadio PCR	74 (55-88)	13	0	8
EcoRI/MseI sites	FAFLP typing (+selective primers)	7 (1-21)	NDd	0	10
EcoRI/MseI sites	FAFLP typing	0 (0-11)	ND	1	10
BamHI/PstI sites	FAFLP typing	0 (0-11)	ND	0	9/9

^aFraction of duplicates showing identical types (31).

^bCI, confidence interval.

^cResults indicated exclude QUB locus 3232.

^dND, not done.