TABLE 3.
Effect of cycling program modifications on product yield
| Amt of template (pg) | Fluorescence intensity of ampliconsa in program:
|
||||
|---|---|---|---|---|---|
| A | B | C | D | E | |
| 65 | − | − | − | − | − |
| 100 | ++ | ++ | +++ | − | ++ |
| 120 | ++ | ++ | +++ | + | ++ |
| 260 | ++ | ++ | +++ | ++ | ++ |
a
The fluorescence intensity of amplicons resulting from program A was arbitrarily designated (++) for all template amounts tested. Relative intensities of amplicons from other PCRs carried out with the same amount of template are indicated as increased (+++) or decreased (+). −, indicates no fluorescence.