TABLE 3.
Comparative analysis of detection of pathogenic mycobacteria in cattle-derived samples by N-PCR and by standard identification tests
| Sample serial no. | Pathogenic mycobacterium(a) detected byd:
|
|||||
|---|---|---|---|---|---|---|
| N-PCR
|
Standard identificationa
|
|||||
| PSLNb | Blood | Milk | PSLN | Blood | Milk | |
| 92 | —e | M. tuberculosis | M. tuberculosis | — | M. tuberculosis | — |
| 104 | M. tuberculosis and M. bovisc | M. bovis | M. bovis | — | — | M. bovis |
| 105 | — | M. bovis | M. tuberculosis | — | — | M. tuberculosis |
| 128 | — | — | M. tuberculosis | — | — | M. tuberculosis |
| 129 | ND | M. tuberculosis | M. bovis | — | M. tuberculosis | — |
| 141 | M. bovis | — | — | M. bovis | — | — |
| 147 | — | — | M. bovis | — | — | M. bovis |
| 149 | — | — | M. bovis | — | — | M. bovis |
| 152 | M. tuberculosis and M. bovis | M. tuberculosis and M. bovis | NA | M. tuberculosis | — | NA |
| 91 | M. tuberculosis | — | M. tuberculosis | — | M. bovis | — |
| 94 | M. tuberculosis | M. tuberculosis | M. tuberculosis | M. bovis | — | — |
| 109 | — | — | M. tuberculosis | — | M. tuberculosis | — |
| 111 | — | — | M. tuberculosis | — | M. tuberculosis | — |
| 113 | — | M. bovis | M. bovis | M. tuberculosis | — | — |
| 134 | — | — | M. bovis | — | M. bovis | |
| 140 | M. bovis | M. tuberculosis | — | — | — | M. tuberculosis |
| 100 | — | — | — | M. tuberculosis | — | M. bovis |
a
Identification of cultivated mycobacterial isolates by standard tests (CDC, Atlanta, Ga.).
b
FNA derived from periscapular lymph gland.
c
M. tuberculosis and M. bovis detected in samples derived from a single animal/in an individual sample.
d
ND, speciation not done. PCR product identified as derived from mycobacteria belonging to the MTC. NA, not available.
e
no mycobacterial pathogen detected or isolated.