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Steady-state and pre-steady kinetics of PTP1B. (A) Steady-state (left) and stopped-flow (right) experiments were performed for WT and allosteric mutants with pNPP as the substrate. (B) Progress curves representing the formation of dephosphorylated peptides as a function of time with WT PTP1B. Black lines are the non-linear least squares fit to the gray data points. The peptide sequences are shown at the top of each panel. Identical experiments for the mutants are shown in the SI.
