Sensitivity analysis of FLiP using purified DNA. Lane M, 100-bp ladder; lanes 1 to 7, dilution series of H37Rv DNA at 10 ng/μl, 1 ng/μl, 100 pg/μl, 10 pg/μl, 1 pg/μl, 100 fg/μl, and 10 fg/μl; lane-K, negative control; lane +K, positive control (M. tuberculosis crude lysate).