TABLE 2.

Interassay reproducibilities of the HCoV OC43 and HCoV 229E qRT-PCRs^a

Sample	Dilution	cRNA copies	Mean CT	SD	CV (%)
HCoV OC43 cRNA	1 × 10−5	2.35 × 108	15.96	0.02	0.14
	1 × 10−6	2.35 × 107	18.67	0.08	0.44
	1 × 10−7	2.35 × 106	22.22	0.02	0.08
	1 × 10−8	2.35 × 105	25.18	0.11	0.44
HCoV 229E cRNA	1 × 10−5	2.26 × 108	17.35	0.03	0.18
	1 × 10−6	2.26 × 107	20.82	0.03	0.13
	1 × 10−7	2.26 × 106	24.19	0.06	0.24
	1 × 10−8	2.26 × 105	27.76	0.07	0.24

^aThe mean values of C_T, standard deviations (SDs), and coefficients of variation (CVs) were determined for three assay runs.