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Optimization of the fusion construction to be used for ELISA serological assay. The reactivity of four well-characterized sera against different concentrations of rC29FL (A) and maltose binding protein (B) was represented. The sera used were previously characterized as highly reactive (A), weakly reactive (B), strongly negative (with low unspecific reactivity) (C), and weakly negative (negative but presenting high background) (D) with respect to their reactivity against TH.
