TABLE 5.
Measures of agreement in HPV detection results between oral rinse samples collected at visit 1 and visit 2, stratified by standardization method
| Cell no. standardized (89 pairs)
|
Vol standardized (89 pairs)
|
Cell no. versus vol standardized measures of agreement
|
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|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Visit 1d | Visit 2d | Measures of agreement
|
Visit 1d | Visit 2d | Measures of agreement
|
Visit 1 (89 pairs)
|
Visit 2 (89 pairs)
|
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| % Agreement (95% CI) | Kappa (95% CI) | % Agreement (95% CI) | Kappa (95% CI) | % Agreement (95% CI) | Kappa (95% CI) | % Agreement (95% CI) | Kappa (95% CI) | |||||
| Oral HPV infection status by individual | ||||||||||||
| HPV negative | 50 (56, 45-67) | 48 (54, 43-65) | 80 (69-88) | 0.59 (0.41-0.77) | 45 (51, 39-61) | 43 (48, 37-59) | 80 (69-88) | 0.60 (0.42-0.78) | 90 (81-96) | 0.80 (0.68-0.92) | 94 (87-99) | 0.89 (0.81-0.97) |
| HPV positivea | 39 (44, 43-55) | 41 (46, 35-57) | 44 (49, 39-60) | 46 (52, 41-62) | ||||||||
| High-risk HPV | 31 (35, 25-46) | 32 (36, 26-47) | 85 (76-92) | 0.68 (0.52-0.84) | 33 (37, 27-48) | 37 (42, 31-53) | 80 (69-88) | 0.58 (0.40-0.76) | 93 (86-98) | 0.85 (0.73-0.97) | 94 (87-99) | 0.88 (0.78-0.98) |
| Low-risk HPV | 15 (17, 9-26) | 19 (21, 13-31) | 84 (75-92) | 0.49 (0.25-0.73) | 18 (20, 12-30) | 20 (22, 14-33) | 84 (75-92) | 0.53 (0.31-0.75) | 92 (84-97) | 0.74 (0.56-0.92) | 94 (87-99) | 0.83 (0.69-0.97) |
| Type-specific infections detected (no.) | ||||||||||||
| High-risk HPV | 45 | 50 | 98 (97-99) | 0.64 (0.52-0.76) | 55 | 70 | 97 (96-98) | 0.59 (0.49-0.69) | 99 (98-100) | 0.83 (0.75-0.91) | 99 (98-100) | 0.79 (0.71-0.87) |
| Low-risk HPV | 17 | 23 | 98 (97-99) | 0.59 (0.31-0.67) | 19 | 27 | 98 (97-99) | 0.51 (0.33-0.69) | 99 (98-100) | 0.77 (0.61-0.93) | 99 (98-100) | 0.80 (0.68-0.92) |
| Total HPVs detected | 62 | 73 | 98 (97-99) | 0.62 (0.52-0.72) | 74 | 97 | 98 (97-99) | 0.57 (0.47-0.67) | 99 (98-100) | 0.82 (0.74-0.90) | 99 (98-100) | 0.79 (0.73-0.85) |
| Human cell no. per PCR | ||||||||||||
| Medianb | 48,100 | 47,580 | 127,523 | 216,500 | ||||||||
| Interquartile rangec | 37,992-50,265 | 22,735-50,400 | 58,400-251,000 | 67,000-485,000 | ||||||||
By McNemar's test, for rinse 1 and rinse 2 for cell number-standardized samples, P = 0.81; for rinse 1 and rinse 2 for volume-standardized samples, P = 0.64; for rinse 1, cell number versus volume standardized, P = 0.18; for rinse 2, cell number versus volume standardized, P = 0.03.
Wilcoxon signed-rank test comparing ERV-3 in rinse 1 versus ERV-3 in rinse 2, P = 0.88 in cell number-standardized samples and P = 0.0008 in volume-standardized samples.
IQR reports the 25th and 75th percentiles and is a measure of data variability.
Unless otherwise indicated, values are numbers of samples (percent, 95% CI).