TABLE 2.
Molecular masses of peptides derived from trypsin digestion of the purified rCts1Ur protein measured by MALDI mass spectrometry compared to the predicted molecular masses of translated peptides of CTS1
| Translated CTS1 sequence (start-end) | Molecular mass (Da)
|
|
|---|---|---|
| Predicted | Measured | |
| SYPVPEAPAEGGFR (26-39) | 1,476.71 | 1,476.42 |
| SVVYFVNWAIYGR (40-52) | 1,573.81 | 1,573.56 |
| HYPGDKWDEPGK (92-112) | 1,428.65 | 1,428.58 |
| TLLSIGGWTYSPNFK (124-138) | 1,683.87 | 1,683.66 |
| KFLLTIASPAGPQNYNK (205-221) | 1,862.02 | 1,861.84 |
| VSGHMSNVFPSTTKPESTPFSSDK (248-271) | 2,583.20 | 2,583.80 |
| IVLGMPLYGR (286-295) | 1,134.63 | 1,134.42 |
| YLISYDTVK (350-358) | 1,101.56 | 1,101.54 |
| TGNESLVGTVVNGLGGTGK (385-403) | 1,759.91 | 1,760.04 |