TABLE 3.
Confirmation of equivocal IFA test results with ELISA and Western blot assay
| Subtype | Test | Result for sample no. with serum origin:
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Confirmed Q fever patients
|
Japanese pneumonia patients
|
||||||||
| 1 | 2 | 3 | 1a | 2 | 3 | 4b | 5b | ||
| IgM | IFAc | 32 | <16 | <16 | 32 | 32 | 32 | 64 | 64 |
| ELISAd | + | − | − | + | − | − | − | − | |
| WBAe | + | − | − | + | − | − | + | + | |
| IgG | IFA | 128 | 128 | 128 | <16 | <16 | <16 | 128 | 128 |
| ELISA | + | + | + | − | − | − | − | − | |
| WBA | + | + | + | − | − | − | − | − | |
a
Probable acute case.
b
Suspected acute case.
c
The numbers show titers as twofold dilutions.
d
+, positive, if the index was more than 11 units; −, negative, if the index was less than 9 units.
e
+, positive, if sera recognized the approximately 27-kDa protein; −, negative, if sera did not recognize the protein.