TABLE 4.
Confirmation of equivocal ELISA results with IFA test and Western blotting assay
| Subtype | Test | Result for indicated sample no. with serum origin:
|
|||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Confirmed Q fever patients
|
Japanese clinical specimen
|
||||||||||||||||||
| 1 | 2 | 3 | 4 | 1a | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | ||
| IgM | ELISAb | 15.1 | 13.8 | 8.3 | 8.8 | 12.8 | 14 | 12.5 | 3.4 | 7.3 | 7.9 | 10.4 | 4.2 | 8.3 | 13.5 | 11.1 | 11.2 | 15.7 | 5.1 |
| IFAc | 32 | + | <16 | <16 | 32 | <16 | <16 | <16 | <16 | <16 | <16 | <16 | <16 | <16 | <16 | <16 | <16 | <16 | |
| WBd | + | + | − | − | + | − | − | − | − | − | − | − | − | − | − | − | − | − | |
| IgG | ELISA | 14.7 | 14 | 15.1 | 14.7 | 9.7 | 4.3 | 5.2 | 15.8 | 13 | 14.8 | 11.9 | 19.4 | 14.1 | 4.1 | 2.7 | 4.5 | 4.6 | 12.2 |
| IFA | 128 | + | 128 | + | <16 | 32 | <16 | <16 | <16 | <16 | <16 | <16 | <16 | <16 | <16 | <16 | <16 | <16 | |
| WB | + | + | + | + | − | − | − | − | − | − | − | − | − | − | − | − | − | − | |
a
Probable acute case.
b
The numbers indicate the ELISA index.
c
The numbers indicate the IFA titer of the diluted sera (see details in the text).
d
+, positive; −, negative in the recognition of the approximately 27-kDa protein.