FIG. 3.
Effect of gpb1Δ gpb2Δ mutations is independent of adenylyl cyclase and the high-affinity cAMP phosphodiesterase. Tenfold dilutions of the following strains were spotted onto medium containing the indicated concentrations of cAMP: HS222-3A (cyr1Δ pde2Δ) and HS224-3D (gpb1Δ gpb2Δ cyr1Δ pde2Δ) grown in synthetic complete medium containing 2 mM cAMP (upper panel), and HS222-3A.T (cyr1Δ pde2Δ) carrying vector YEp352 or plasmid YCp50-RAS2ala18val19 grown in medium lacking uracil and containing 2 mM cAMP (lower panel). Plates were photographed after 2 days of growth.