FIG. 4.
Analysis of H99 and chitin synthase deletion strains for in vitro sensitivity to cell wall stressors. Strains were grown in YPD medium to mid-log phase, serially diluted 10-fold (starting with 1 × 105 cells in the left column of each panel), and plated on YPD solid medium and YPD solid medium supplemented with various cell wall stressors. Strains plated on YPD medium were also tested for high-temperature sensitivity. Time of incubation is indicated at the far left. Strains are indicated at the left of the panels. Incubation conditions are indicated at the top of the panels. All plates containing cell wall stressors were incubated at 30°C.