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The N-terminal arm of Mcm1 is required for the regulation of a specific subset of salt-induced and cell wall genes. The mRNA levels of genes with putative Mcm1-binding sites were measured under low- and high-salt conditions by Northern blot analysis of strain JM01 transformed with either wild-type MCM1 (pDA135) or mcm1-Δ2-17 (pDA136). The CAR2 and CLB2 genes are regulated by Mcm1 but do not require the NT arm for activation. ACT1 is shown as a loading control.
