FIG. 5.
(A) The T35A mutation in Mcm1 suppresses the growth defect of Mcm1-Δ2-17 on media containing CFW, pH 8.5, and CaCl2. Serial dilutions (threefold) of yeast strain JM01 transformed with plasmids containing MCM1, mcm1-Δ2-17, mcm1-T35A, or mcm1-Δ2-17,T35A were spotted onto the indicated media and grown at 30°C for 2 to 5 days. (B) The T35A mutation does not suppress the defective YGP1 transcription of Mcm1-Δ2-17. Northern blots are shown for the expression of YGP1 and ACT1 (loading control) for strains containing wild-type MCM1 and the indicated mcm1 mutants.