TABLE 1.
Plasmids used in this study
| Plasmid | Description | Reference or source |
|---|---|---|
| pJM231 | MCM1 CEN HIS3 | 1 |
| pDA126 | mcm1-Δ2-17 CEN HIS3 | This study |
| pDA135 | C-terminally-V5 tagged MCM1 CEN HIS3 | This study |
| pDA136 | C-terminally-V5 tagged mcm1-Δ2-17 CEN HIS3 | This study |
| pDA135-T35A | C-terminally-V5 tagged mcm1-T35A CEN HIS3 | This study |
| pDA181 | 700 bp of YGP1 promoter lacZ reporter | This study |
| pDA181-r1 | pDA181 with mutation in site R1 | This study |
| pDA181-m2 | pDA181 with mutation in site M2 | This study |
| pDA181-m3 | pDA181 with mutation in site M3 | This study |
| pDA181-m4 | pDA181 with mutation in site M4 | This study |
| pDA181-r1m2 | pDA181 with mutation in sites R1, M2 | This study |
| pDA181-m2m3 | pDA181 with mutation in sites M2, M3 | This study |
| pDA181-m1m2m3 | pDA181 with mutation in sites M1, M2, M3 | This study |
| pDA181-r1m2m3 | pDA181 with mutation in sites R1, M2, M3 | This study |
| pDA181-m1m2m3m4 | pDA181 with mutation in sites M1, M2, M3, M4 | This study |
| pDA181-r1m1m2m3 | pDA181 with mutation in sites R1, M1, M2, M3 | This study |
| pDA192 | R1M2M3 site cloned into pGD579 | This study |
| pTBA22 | Mcm1-18-96 fused to maltose-binding protein | 2 |
| pTBA25 | Mcm1-1-96 fused to maltose-binding protein | 2 |