Table 1.

Oligonucleotide Primers and Conditions

	Forward Primer		Reverse Primer
AnalysisandExon	Namea	Sequence(5′–3′)	Namea	Sequence(5′–3′)	AnnealingTemperature(°C)	MgCl2(mM)b
DGGE:
1	ABCR1-For	AATCTGGTCTTCGTGTGGTC	ABCR1-GC	GTTTATTTGCTCCACACCTC	58	1.0+
2	ABCR2-GC	AATCTCTTAGCACCACTGAAC	ABCR2-Rev	AGGCCCAGACCAAAGTCTC	58	1.0−
3	ABCR3-For	CCTGCTTGGTCTCCATGAC	ABCR3-GC	ACGTGAAGGGGTGTGCAAC	57	1.0+
4	ABCR4-GC2	CCTTATTAATGAGGCTTTGTC	ABCR4-Rev2	ATAGGTGAGGGAAATGATGC	57	1.5+
5	ABCR5-GC	CCATTTCCCCTTCAACACCC	ABCR5-Rev	GTGCTTCCCTCCCCTCCAG	58	1.0+
6	ABCR6-For	CTACCACAGGGCAGTTTCTA	ABCR6-GC	CAGGAATCACCTTGCAATTG	58	1.0+
7	ABCR7-GC	GATCAGACTG TGCCTATGTG	ABCR7-Rev	ATAAGTGGGGTAAATGGTGG	57	1.0+
9	ABCR9-GC	AGGTTACAAGCAATGGGGAG	ABCR9-Rev	TCTGGGAGGTCCAGGGTAC	58	1.0−
12	ABCR12-For	AGTTGAGTCTTTGCAGTTGG	ABCR12-GC	CTGACTTTGGAGAAATGCAG	58	1.5+
13	ABCR13-GC	TCGGGAGGTGTGAGTGAGC	ABCR13-Rev	TTAGCGTGTCATGGAGGAGG	58	1.0+
14	ABCR14-GC2	ATTCTGCCTCTACCAGGTAC	ABCR14-rev	AATCCAGGCACATGAACAGG	57	1.5+
15	12-For	AGGCTGGTGGGAGAGAGC	ABCR15-GC	GGACTGCTACGGACCATTC	56	1.5+
16	13-For	CTGTTGCATTGGATAAAAGGC	ABCR16-GC	GATGAATGGAGAGGGCTGG	56	1.5−
17	Exon J-For	CTGCGGTAAGGTAGGATAGGG	ABCR17-GC	CACACCGTTTACATAGAGGGC	58	1.5+
18	Exon K-For	CTCTCCCCTCCTTTCCTG	ABCR18-GC2	GCCTTTTCCTCGCCTCTG	56	1.0+
19	15-For	TGGGGCCATGTAATTAGGC	ABCR19-GC	TGGGAAAGAGTAGACAGCCG	57	1.5−
19	ABCR19-For2	AAGATTTTTGAGCCCTGTGG	ABCR19-GC	TGGGAAAGAGTAGACAGCCG	57	1.5+
20	ABCR20-GC	GCCCTCCTAAGGCATGTTG	2F3-Rev	TATCTCTGCCTGTGCCCAG	57	1.0+
21	2R5N-For	GTAAGATCAGCTGCTGGAAG	ABCR21-GC	GAAGCTCTCCTGCTCCAAGC	58	1.0+
22	2F5R-For2	AGGTACCCCCACAATGCC	ABCR22-GC	AGCCCAGCCCAGGAGACT	56	1.0+
23	ABCR23-GC	TTTTTGCAACTATATAGCCAGG	2F6-Rev	AGCCTGTGTGAGTAGCCATG	58	1.5−
24	2F7R-For	GCATCAGGGAGAGGCTGTC	ABCR24-GC	CCAGACGGAACCCAAGTATG	59	1.0+
25	ABCR25-GC	GGTAACCTCACAGTCTTCC	23F1-Rev	GGGAACGATGGCTTTTTGC	56	1.5−
26	ABCR26-GC2	TCCCATTATGAAGCAATACC	ABCR26-Rev	CCTTAGACTTTCGAGATGG	48	1.5+
28	ABCR28-GC	ACGTGTGACATCTCCATGCC	ABCR28-Rev	CCCTTCTAAGCAGCATGTGA	58	1.0+
29	ABCR29-GC	AGGCTCTGAGTTGCATGATG	ABCR29-Rev	CTGCCATCTTGAACCCACC	59	1.0+
31	ABCR31-For	TATAAGTCCTCAAGTTCCAAG	ABCR31-GC	AATATCTTCTACAGGGAGCC	56	1.5+
32	ABCR32-For	TAACGGCACTGCTGTACTTG	ABCR32-GC	TCATGGCTGTGAGGTGTGC	58	1.0+
33	33G1-For	TTCATGTTTCCCTACAAAACCC	ABCR33-GC	AAAATCCTACTCAAATCTCCAG	58	1.5−
34	ExA-For	GCTTAACTACCATGAATGAG	ABCR34-GC	TCAGCAGGAGGAGGGATG	56	1.0−
35	ABCR35-GC	TAACTAGCTGTTAATGCAGCG	ExB-Rev	AAGAGTGGAGAAGGTGACAA	58	1.5−
36	ABCR36-GC	GTATCTTCTCCTCCTTCTGC	ABCR36-Rev	CACACAAGCTCCACCTTGG	58	1.5+
37	ABCR37-GC	CAGGTCTGAGAGGTTAAGTG	ABCR37-Rev	CCACCAGGCTTCTCTTCAG	58	1.0+
39	ABCR39-For2	GGTTTGCCCCGTTTCCAAC	ABCR39-GC	TCCCAGCTTTGGACCCAG	56	1.0+
40	ABCR40-GC	AGGTCTGTGGGGTGAGCTG	ABCR40-Rev	TCTGGATGCCCTGAGCTGC	58	1.0+
41	ABCR41-For	GAAAGGACAGTGCCAAGGAC	ABCR41-GC	TCTAACCAGCACCTCCAAAC	58	1.5+
42	ABCR42-GC	CCGTCTCAGTTCTCAGTCC	ABCR42-Rev	AGAGCTGATGTTCGGAAGCC	57	1.0+
43	4RX-For	CTTACCCTGGGGCCTGAC	ABCR43-GC	TCAGAGCCACCCTACTATAG	56	1.5+
45	ABCR45-GC	CTTGTCTTCTCCAAATGGCA	ABCR45-Rev	TTTAAGCCCTTGGTGCGGC	51	1.0+
46	E1R-For	GAAGCAGTAATCAGAAGGGC	ABCR46-GC	CCTCACATTCTTCCATGCTG	57	1.0+
47	ABCR47-For	CACATCCCACAGGCAAGAG	ABCR47-GC	ATCCACAGAAGGCAACAAGG	57	1.0+
48	ABCR48-GC	AGGCCCAACCACTAACAGAG	E3F-Rev	ACACTGGGTGTTCTGGACC	57	1.0+
50	ABCR50-GC	AAACCAAGATGACGCGAGTC	ABCR50-Rev	GGAACGAGCGGTGTGAAAG	57	1.0+
dHPLC:
8	ABCR8F2	GAGCATTGGCCTCACAGCAG	ABCR8R2	CCCCAGGTTTGGTTTCACC	54	1.0−
27	ABCR27F2	GAGATCCAGACCTTATAGGC	23F3R	GTTATAACCCATGCCTGAAG	54	1.5−
30	ABCR30F2	ACTTTGAGGCTGATTATGGAA	ABCR30R	CCCCGTTGTTTGGAGGTC	54	1.5−
38	ABCR38F2	GGAATGGAATGTGGAACTCC	ABCR38R2	ACACATACTCTACTATCCTAC	54	1.5−
44	62r4F	GAAGCTTCTCCAGCCCTAGC	62r3R	TGCACTCTCATGAAACAGGC	54	1.0−
49	ABCR49F	GTGTAGGGTGCTGTTTTCC	ABCR49R	CAAGCTGTGGACTGCATAAG	54	1.5−
SSCP:
10	ABCR10F4	ATCTTTGTCTGGTTTTAGGC	ABCR10R4	CCCCCCTTACTCTGATCAT	50	1.5−
11	ABCR11F2	GAATTTCTAAGCAGAGCAGTG	ABCR11R	AGCTCTGGCCCCACTCATG	54	1.5−

^aGC = GC clamp at the 5′ end of the respective primer.

^bPCR reaction with (+) or without (−) 4% formamide.