Table 2.

Primers for Amplification and Sequencing of SLC17A5 Exons

Exon and PCR and Sequencing Primers	AmplifiedProduct(bp)	AnnealingTemperature(°C)
2:
Forward, 5′-AGGAGTTCAAGACCAGCCTAAGCAACATG-3′	370	54
Reverse, 5′-TACACAAACAAAGTATAGTTTCTGTAGGATGA-3′
3:
Forward, 5′-AATCTCATTTATATGTACTTACATGCCAGT-3′	415	53
Reverse, 5′-GGTTCATATTCATACCAAAGAATGACATC-3′
4:
Forward, 5′-CATGGAACCTGATTCGTTTGATTCTTATC-3′	310	58
Reverse, 5′-AACATTGCATCGTTCTGGTATGCAGGCC-3′
5:
Forward, 5′-CCCATCCTCTGTAAGCAGTAGACTTGTC-3′	300	58
Reverse, 5′-CAGGAAGTGTTTCAGAACTGTGGTTCAG-3′
6:
Forward, 5′-TCAAGACATGTAAAAATTGTTGTTGGTGC-3′	345	54
Reverse, 5′-CTACTTTGTCTAGAGCATGCACAATAGG-3′
7:
Forward, 5′-GAATTCTGGATAGAGTTTTAGCTGATACC-3′	420	53
Reverse, 5′-AGGACAGCAGAGTAAAATGGAAGATACAG-3′
8:
Forward, 5′-TTCAAGTGGTGGTCATTGGCTGTGATGA-3′	345	58
Reverse, 5′-GTGATTAGAGCGAGGTGTTCTGTGTTGAG-3′
9:
Forward, 5′-GTCTTCCAGGCAATATAGTGTTTCTACAG-3′	390	56
Reverse, 5′-AAGTGCTGGGATTACAGGTGTGAGTCACC-3′
10:
Forward, 5′AGGCTGCAGTGAGCTGAGATCATCCCAC-3′	380	55
Reverse, 5′-ATACTTTCCCATCCATTAAGGCATTTAGC-3′
11:
Forward, 5′-ATGTGGCTGTTTTAACACTCTTGTGACAC-3′	330	64
Reverse, 5′-ATAGAATGCTTACACAATACAGAAGGCAC-3′