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. 2005 Nov 18;1(3):e30. doi: 10.1371/journal.ppat.0010030

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Copyright: © 2005 Hohl et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Absolute number of BAL cells, total neutrophils in BAL, and total macrophages in BAL for C57BL/6 mice 24 h after intratracheal instillation with either heat-killed swollen conidia or live conidia. The bar graphs show the average cell numbers + standard deviation from four mice per group.

(B) Flow cytometric analysis of live BAL cells stained for Ly6G and CD11c as in Figure 1B.

(C) A. fumigatus conidia were allowed to initiate germination for 3, 5, or 7 h, heat-killed, and added to BMMØ cultures for 18 h. TNF-α/MIP-2 concentrations in the supernatants were determined by ELISA. The bar graphs represent the average ± standard deviation of three wells per condition. One of four experiments is shown.