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. 2005 Nov 25;1(5):e69. doi: 10.1371/journal.pgen.0010069

Figure 1. Respiration Is Not Required for Life Span Extension by CR in BY4742.

Figure 1

(A) BY4742 rho0 strains lack mitochondrial DNA. DAPI staining of BY4742 (i) wild-type or (ii) rho0 cells grown under standard conditions (2% glucose) and calorie-restricted (iii) wild-type or (iv) rho0 cells (CR = 0.05% glucose).

(B) BY4742 rho0 strains are unable to grow on glycerol as the sole carbon source. (i) BY4742 wild-type or (ii) rho0 cells on YEP supplemented with 2% glucose or 3% glycerol.

(C) CR increase life span in BY4742 rho0 cells. Replicative life span analysis for BY4742 wild-type and rho0 cells on 2% glucose and 0.05% glucose (CR). Mean life span is shown in parentheses.